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In particular, during NTERA2 differentiation, A2B5 expression has been utilized to obtain a cell population that possesses high levels of NeuroD1 and NSE expression, in comparison to A2B5-negative cells [Przyborski et al, 2000] Another surface marker, NCAM, is a cell surface glycoprotein believed to be involved in neural cell cell adhesion [Rutishauser et al, 1982], and antibodies to N-CAM provide useful tools for identifying cells of neural lineage Other markers, which are intracellular, are not so useful for purifying cell populations but are nevertheless invaluable for monitoring the progress of neural differentiation One such marker is the class VI intermediate lament protein nestin, which is expressed during neurogenesis [Hock eld et al, 1985] and in adult neuroepithelial stem cells [Lendahl et al, 1990] It was rst identi ed in rat with the Rat-401 monoclonal antibody [Hock eld et al.

Proposition 3.8: An n n matrix A has as inverse matrix A 21 (modulo m) if and only if the rows of A are linearly independent over Zm. Proposition 3.9: If the n n A has an inverse modulo m, Gaussian elimination will succeed if and only if the m columns of X and Y contain n linearly independent vectors modulo m.

You can download the K-Xploit software from http://www.psp-hacks.com/ psp-kxploit-howto.php, or from the official Hacking the PSP Web site (www .hackingpsp.com). Once you ve downloaded the software, and found the homebrew applications you want to install, install the K-Xploit software. Plug in your PSP and put it in USB Connection mode ( host mode ), so the program can automatically install the software on your PSP. Once your PSP is in host mode, launch the K-Xploit software. You will be greeted with a screen similar to the one in Figure 19-1.

, 1985] and has been localized to many areas, such as the subventricular zone [Doetsch et al, 1997], the hippocampal dentate gyrus [Yamaguchi et al, 2000], and even the pancreas [Street et al, 2004] It is mainly expressed in neuroepithelial progenitor cells but is also seen in other cell types such as post-injury astrocytes [Clarke et al, 1994] and is downregulated in mature neurons and mature oligodendrocytes [Gallo et al, 1995] However, nestin is not an ideal marker for the identi cation of neural progenitors since it is abundantly expressed by undifferentiated ES and NTERA2 cells -Tubulin III (also called -6 tubulin) is another cytoskeletal protein that is found exclusively in neurons of higher vertebrates (except for transient expression in some embryonic structures) and is a marker of neuronal differentiation and neurite outgrowth [Schachner et al, 1981], [see for review Katsetos et al.

The Stamp tool can help you place objects onto an organic surface. The Stamp tool helps imbed the selected item into terrain by adjusting the geometry to enfold the object (Fig. 12-12 through Fig. 12-14).

In most database programs, the process of performing a mail merge involves two steps: exporting the merge data from a database program and then merging it with a word processing document that includes field placeholders (to show where the merge data should be inserted). Although you can also do it this way with FileMaker Pro, you can perform a merge without leaving FileMaker by embedding one or more merge fields in any text block.

, 2003] The monoclonal antibody TUJ1 is speci c for this isotype and binds to the C-terminus, allowing immunostaining [Lee et al, 1990] A series of studies on the highly conserved gene Musashi-1 have shown that it plays an important role in cell fate decision, including the maintenance of the stem cell state, differentiation, and tumorigenesis [Okano et al, 2005] It has also been used as a marker for neural stem and progenitor cells in the human brain [Okano et al, 2005] Rat monoclonal antibodies (Mab 14H1 and 14B8) that recognize Musashi-1 but not the Musashi-1-related protein Musashi-2 have been generated [Kanemura et al, 2001] Oligodendrocytes can be detected by use of the mouse IgM monoclonal antibodies O1 O4, which exclusively detect cells of oligodendrocyte lineage but not neurons, astrocytes, or broblasts [Sommer et al, 1981].

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Cells expressing these antigens can be classi ed as those found only on galactocerebroside-expressing oligodendrocytes, in the case of O1 and O2, or those that are present on both galactocerebroside-positive (usually after prolonged time in culture) and -negative cells, in the case of O3 and O4 [Schachner et al, 1981] O3 and O4 are expressed a day earlier than O1 and O2 in cells cultured from the mouse pons, and they are also detected at birth in mouse cerebellum, as opposed to O1 and O2, which do not appear until 1 week postnatally [Schachner et al, 1981]..